TB-500 Spray

Format

TB-500 Spray is supplied as a ready-to-use buffered aqueous solution in a 10 mL spray bottle, so no reconstitution step is needed. Contents: 10 mg TB-500 per 10 mL.

Identity

TB-500 is a synthetic peptide corresponding to the actin-binding region of thymosin beta-4, a 43-residue G-actin-sequestering protein found in most mammalian cells. The supplied material is the acetylated fragment built around the LKKTETQ motif at residues 17-23 of the parent sequence, which is the portion that carries actin-binding activity.

Molecular target

No cell-surface receptor has been established for this fragment. The LKKTETQ motif binds monomeric G-actin directly, forming a one-to-one complex, so the target is a cytoskeletal protein rather than a membrane receptor.

Signalling pathway

Sequestering G-actin shifts the equilibrium between monomeric and filamentous actin. Published work has examined the downstream consequences for cytoskeletal reorganisation, directed cell migration and the transcription of matrix and angiogenic genes, including MRTF/SRF-dependent transcription that is itself sensitive to the free G-actin pool.

Research context

Endothelial, fibroblast and cardiomyocyte culture, and rodent injury models. In vitro and preclinical evidence only.

Specification

• Form: ready-to-use buffered aqueous solution, 10 mL spray bottle
• Contents: 10 mg TB-500 per 10 mL
• Purity: each compound verified by high-performance liquid chromatography with mass spectrometric confirmation
• Analysis: third-party tested
• Storage: 2-8 °C, keep sealed and upright

For laboratory research use only. Not for human or veterinary use.